SARS-CoV/SARS-CoV-2 Spike antibody,Chimeric MAb

Product name

SARS-CoV / SARS-CoV-2 spike antibody, chimeric MAb

Validated applications: ELISA , Neutralization

Application Notes

IHC, FCM, IF, IP et al. the applications have not been validated. (Antibody applications have not been validated with corresponding virus-positive samples. Optimal concentrations/dilutions must be determined by the end-user).

Specificity

Peak of SARS coronavirus

  • It is cross-reactive in ELISA with

SARS-CoV Peak S1 Protein (Cat # 40150-V08B1)
SARS-CoV-2 Spike S1 Protein (2019-nCoV) (Catalog # 40591-V08H)
SARS-CoV-2 (2019-nCoV) RBD protein spike (Cat # 40592-V08B)

  • No cross-reactivity in ELISA with

MERS-CoV Spike S1 Protein (Cat # 40069-V08B1)
HCV-HKU1 (isolate N1) Spike S1 protein (Cat # 40021-V08H)
HCV-HKU1 (isolate N5) Spike S1 protein (Cat # 40602-V08H)
Spike S1 HCoV-NL63 Protein (Cat # 40600-V08H)
Spike S1 HCV-229E Protein (Cat # 40601-V08H)
HCV-OC43 Spike S1 + S2 ECD Protein (Cat # 40607-V08B)

Immunogen

Recombinant SARS-CoV Spike RBD Protein (Catalog # 40150-V08B2)

Preparation

It is a chimeric monoclonal antibody that combines the constant domains of the human IgG1 molecule with mouse variable regions. The variable region was obtained from a mouse immunized with purified recombinant SARS-CoV peak RBD protein. The antibody was produced using recombinant antibody technology.

Source

Mouse (variable region) / human (kappa constant / IgG1) monoclonal antibody Clone # D002

Purification: Protein A

Formulation: 0.2 μm filtered solution in PBS

Conjugated: Unconjugated

Form: Liquid

Shipping

This antibody is shipped as a liquid solution at room temperature. Upon receipt, please store immediately at the recommended temperature below.

Storage

This antibody can be stored at 2-8 ° C for one month without detectable loss of activity. Antibody products are stable for twelve months from the date of receipt when stored between -20 ℃ and -80 ℃. Without condoms. Avoid repeated freeze-thaw cycles.

Notes

Microneutralization (MN):

Neutralization activity is measured by an in vitro microneutralization assay. The virus microneutralization (MN) test was performed on 293T-ACE2 cells infected with the SARS-CoV-2 Spike pseudovirus (2019-nCoV) under treatment with serial dilutions of neutralizing antibody. The infection was neutralized by increasing the concentrations of Anti-SARS-CoV-2 Neutralizing Antibody (Catalog # 40150-D001). The inhibition rate was determined by comparing the relative light unit (RLU) of the luciferase reporter at different antibody concentrations. The IC50 is typically 46.762 µg / ml.

Leave a Reply

Vaša adresa e-pošte neće biti objavljena. Obavezna polja su označena sa * (obavezno)

Related Post

Isotype-Specific Fc Effector Functions Enhance Antibody-Mediated Rift Valley Fever Virus Protection In Vivo

Funkcije Fc efekta specifične za izotip Poboljšavaju zaštitu od virusa groznice doline pukotina posredovane antitijelima in vivoFunkcije Fc efekta specifične za izotip Poboljšavaju zaštitu od virusa groznice doline pukotina posredovane antitijelima in vivo

Pronađen 1931. godine, virus groznice Rift Valley Valley (RVFV) arbovirus je koji uzrokuje bolesti kod ljudi i stoke. Kod ljudi, bolest se kreće od samoograničavajuće febrilne bolesti do ekstremnog hepatitisa ili encefalitisa. Trenutačno

Anti Human IREM-2Anti Human IREM-2

Properties Clone: UP-H2 Antibody type: primary antibodies Isotype: mouse IgG1κ Applications: FC Alternative names: CD300LE, CLM-2, CMRF35-A5, IREM-2, PIgR-2, LMIR6 Conjugate: APC Recommended dilution: 1:50 Remark: Cells must be stained