Corticosterone ELISA Kit
Detection method: Colorimetric
Cell culture supernatant, saliva, milk, urine, serum, plasma
Test type: Competitive
Sensitivity: = 0.3 ng / ml
0.391 ng / ml – 100 ng / ml
Recovery: = 101%
Test time: 3 h 00 min
Standard multi-step test
Reacts with: Mouse, Rat, Human, Baboon
The Corticosterone ELISA Kit is a competitive enzyme-linked immunosorbent assay designed for the quantitative measurement of corticosterone levels in plasma, serum, urine, milk, saliva, and cell culture supernatant. A specific corticosterone antibody has been precoated in 96-well plates and blocked. Standards or test samples are added to the wells and then biotinylated corticosterone is added and then washed with wash buffer. The avidin-biotin-peroxidase complex is added and unbound conjugates are washed with wash buffer.
Then TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue product that changes to yellow after adding an acid stop solution. The density of the yellow staining is inversely proportional to the amount of corticosterone captured on the plate. Note that the concentrations of the kit components are lot-specific and the end-user should always refer to the vial label.
Store at -20 ° C. See protocols.
Corticosterone is the adrenal steroid, the main glucocorticoid. Glucocorticoid hormones are also known as corticosteroid hormones and are mainly synthesized in the adrenal cortex; however, more recently enzymes involved in its synthesis have been found in a variety of cells and tissues, including the heart. The effects of these hormones are mediated by both cytoplasmic mineralocorticoid receptors (MR) and glucocorticoid receptors (GR), which act as a ligand-inducible transcription factor. Corticosterone has a profound effect on the structure and function of the hippocampus. The action of cerebral corticosterone through the glucocorticoid receptor may involve memory storage. Emotional stress can cause increases in plasma corticosterone.