Corticosterone Enzyme Immunoassay Kit

Overview

Product name

Corticosterone ELISA Kit

Detection method: Colorimetric

Example type

Cell culture supernatant, saliva, milk, urine, serum, plasma

Test type: Competitive

Sensitivity: = 0.3 ng / ml

Distance

0.391 ng / ml – 100 ng / ml

Recovery: = 101%

Test time: 3 h 00 min

Trial duration

Standard multi-step test

Species reactivity

Reacts with: Mouse, Rat, Human, Baboon

Product description

The Corticosterone ELISA Kit is a competitive enzyme-linked immunosorbent assay designed for the quantitative measurement of corticosterone levels in plasma, serum, urine, milk, saliva, and cell culture supernatant. A specific corticosterone antibody has been precoated in 96-well plates and blocked. Standards or test samples are added to the wells and then biotinylated corticosterone is added and then washed with wash buffer. The avidin-biotin-peroxidase complex is added and unbound conjugates are washed with wash buffer.

Then TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue product that changes to yellow after adding an acid stop solution. The density of the yellow staining is inversely proportional to the amount of corticosterone captured on the plate. Note that the concentrations of the kit components are lot-specific and the end-user should always refer to the vial label.

Platform: Microplate

Properties

Storage instructions

Store at -20 ° C. See protocols.

Relevance

Corticosterone is the adrenal steroid, the main glucocorticoid. Glucocorticoid hormones are also known as corticosteroid hormones and are mainly synthesized in the adrenal cortex; however, more recently enzymes involved in its synthesis have been found in a variety of cells and tissues, including the heart. The effects of these hormones are mediated by both cytoplasmic mineralocorticoid receptors (MR) and glucocorticoid receptors (GR), which act as a ligand-inducible transcription factor. Corticosterone has a profound effect on the structure and function of the hippocampus. The action of cerebral corticosterone through the glucocorticoid receptor may involve memory storage. Emotional stress can cause increases in plasma corticosterone.

Leave a Reply

Vaša adresa e-pošte neće biti objavljena. Obavezna polja su označena sa * (obavezno)

Related Post

Influence of Leukotrienes A4 and B4 on the Immunoglobulin Isotype Selection of Normal and Glucocorticoid-Treated Peripheral Blood Mononuclear Cells

Utjecaj leukotriena A4 i B4 na odabir izotipa imunoglobulina normalnih mononuklearnih stanica periferne krvi tretiranih glukokortikoidimaUtjecaj leukotriena A4 i B4 na odabir izotipa imunoglobulina normalnih mononuklearnih stanica periferne krvi tretiranih glukokortikoidima

Pokazujemo da LTA4 (10-100 ng/ml) i LTB4 (10-100 ng/ml) utječu na diferencijaciju B stanica i zamjenu izotipa u jedinstvenu diplomu. Za razliku od LTB4, LTA4 je selektivno pojačao sintezu IgG B stanica

Isotype-Specific Fc Effector Functions Enhance Antibody-Mediated Rift Valley Fever Virus Protection In Vivo

Funkcije Fc efekta specifične za izotip Poboljšavaju zaštitu od virusa groznice doline pukotina posredovane antitijelima in vivoFunkcije Fc efekta specifične za izotip Poboljšavaju zaštitu od virusa groznice doline pukotina posredovane antitijelima in vivo

Pronađen 1931. godine, virus groznice Rift Valley Valley (RVFV) arbovirus je koji uzrokuje bolesti kod ljudi i stoke. Kod ljudi, bolest se kreće od samoograničavajuće febrilne bolesti do ekstremnog hepatitisa ili encefalitisa. Trenutačno