Anti Human IREM-2

Properties

Clone: UP-H2

Antibody type: primary antibodies

Isotype: mouse IgG1κ

Applications: FC

Alternative names: CD300LE, CLM-2, CMRF35-A5, IREM-2, PIgR-2, LMIR6

Conjugate: APC

Recommended dilution: 1:50

Remark: Cells must be stained before fixation if formaldehyde is used as a fixative.

Tested: QC tested

Products used: 130-122-225, 130-122-216

Protocols:

Cell surface flow cytometry staining protocol (PBS / EDTA / BSA 1:50)

Description:

The monoclonal antibody UP-H2 recognizes human IREM-2 (immune receptor expressed in cells derived from monocytes) also known as LMIR6, and unofficially CD300E and CD300LE. It is a member of the CMRF-35 family. This 34 kDa Ig-containing receptor is an activating receptor and, through its charged residues in the cytoplasmic domain, interacts with ITAM-containing adapter proteins such as DAP12.

Expression is found exclusively in monocytes and myeloid DCs. The expression may be transient as monocyte differentiation leads to downregulation of IREM-2, but the expression is found in mature developing countries. Myeloid cell lines such as THP-1 and U937 have also been shown not to express IREM-2. The in vitro addition of UP-H2 antibody, either soluble or cross-linked, stimulates monocytes.

Informed Applications: This UP-H2 antibody has been reported to be used in flow cytometric analysis.

Proven Applications: This UP-H2 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at the rate of 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that stains a cell sample in a final volume of 100 µL. The number of cells must be determined empirically, but can range from 10 ^ 5 to 10 ^ 8 cells/test.

Excitation: 633-647 nm; Emission: 660 nm; Laser: red laser.

Filtration: 0.2 µm post-fabrication filtering.

Destination information

CD300e is a member of the glycoprotein family of cell surface proteins expressed in myeloid cells. CD300e interacts with the tyrosine kinase-binding protein TYRO protein and is believed to participate as an activating receptor. CD300e is expressed on mature myeloid dendritic cells and monocytes and is down-regulated on immature dendritic cells. Additionally, CD300e interacts with DAP-12, which mediates activation signals. The alloreactive response of naïve T cells has been reported to increase with activation of CD300e. Diseases associated with CD300e dysfunction include tungiasis and lice infestation.

Leave a Reply

Your email address will not be published. Required fields are marked *

Related Post

Analysis of IgM, IgA, and IgG isotype antibodies Directed against SARS-CoV-2 spike glycoprotein and ORF8 in the course of COVID-19

Analiza protutijela izotipa IgM, IgA i IgG usmjerenih protiv glikoproteina naraslih SARS-CoV-2 i ORF8 tijekom COVID-19Analiza protutijela izotipa IgM, IgA i IgG usmjerenih protiv glikoproteina naraslih SARS-CoV-2 i ORF8 tijekom COVID-19

Imunološki testovi su običan dijagnostički uređaj koji procjenjuje imunitet kod infekcije koronavirusom tipa 2 (SARS-CoV-2) s ekstremnim akutnim respiratornim sindromom . Ipak, imunološki testovi ne prezentiraju pojedinosti o kontaminirajućim antigenima ili unakrsnim reakcijama te bi mogli pokazati netočno

Isotype-Specific Fc Effector Functions Enhance Antibody-Mediated Rift Valley Fever Virus Protection In Vivo

Funkcije Fc efekta specifične za izotip Poboljšavaju zaštitu od virusa groznice doline pukotina posredovane antitijelima in vivoFunkcije Fc efekta specifične za izotip Poboljšavaju zaštitu od virusa groznice doline pukotina posredovane antitijelima in vivo

Pronađen 1931. godine, virus groznice Rift Valley Valley (RVFV) arbovirus je koji uzrokuje bolesti kod ljudi i stoke. Kod ljudi, bolest se kreće od samoograničavajuće febrilne bolesti do ekstremnog hepatitisa ili encefalitisa. Trenutačno